Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3861] to TMPRSS2
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-TMPRSS2 antibody [EPR3861]
See all TMPRSS2 primary antibodies
Description

Rabbit monoclonal [EPR3861] to TMPRSS2
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human TMPRSS2 aa 1-100. The exact sequence is proprietary. This antibody is specific for the full-length form of human TMPRSS2 (54 kDa).
Database link: O15393
Positive control
- WB: LNCaP, Caco 2 and PC3 whole cell lysates. Human lung, heart, brain, colon, prostate, and small intestine tissue lysates. Mouse prostate and kidney tissue lysates. Rat colon and kidney tissue lysates; IHC-P: Human prostatic adenocarcinoma and human kidney.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3861
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution

Lane 1 : LnCaP cell lysate
Lane 2 : Human prostate tissue lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Human lung tissue lysate
Lane 5 : Human heart tissue lysate
Lane 6 : Human brain tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 54 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

Lanes 1 - 6: Merged signal (red and green). Green - ab92323 observed at 52 kDa. Red - loading control, mouse anti GAPDH observed at 37kDa.

ab92323 was shown to react with TMPRSS2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab92323 and mouse anti GAPDH overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling TMPRSS2 with purified ab92323 at 1/1000 dilution (0.49 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TMPRSS2 with purified ab92323 at 1/1000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution (unpurified)

Lane 1 : Human prostate lysate
Lane 2 : Human small intestine lysate
Lane 3 : LNCaP (human prostate cancer cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

Predicted band size: 54 kDa
Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution (purified)

Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Human colon lysate
Lane 4 : Human small intestine lysate
Lane 5 : Mouse prostate lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Rat colon lysate
Lane 8 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Unpurified ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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