Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3861] to TMPRSS2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TMPRSS2 antibody [EPR3861]
    See all TMPRSS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3861] to TMPRSS2

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TMPRSS2 aa 1-100. The exact sequence is proprietary. This antibody is specific for the full-length form of human TMPRSS2 (54 kDa).
    Database link: O15393

  • Positive control

    • WB: LNCaP, Caco 2 and PC3 whole cell lysates. Human lung, heart, brain, colon, prostate, and small intestine tissue lysates. Mouse prostate and kidney tissue lysates. Rat colon and kidney tissue lysates; IHC-P: Human prostatic adenocarcinoma and human kidney.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : Human prostate tissue lysate
    Lane 3 : Caco 2 cell lysate
    Lane 4 : Human lung tissue lysate
    Lane 5 : Human heart tissue lysate
    Lane 6 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab92323 observed at 52 kDa. Red - loading control, mouse anti GAPDH observed at 37kDa.

    ab92323 was shown to react with TMPRSS2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab92323 and mouse anti GAPDH overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue sections labeling TMPRSS2 with purified ab92323 at 1/1000 dilution (0.49 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TMPRSS2 with purified ab92323 at 1/1000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution (unpurified)

    Lane 1 : Human prostate lysate
    Lane 2 : Human small intestine lysate
    Lane 3 : LNCaP (human prostate cancer cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Western blot - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution (purified)

    Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Human colon lysate
    Lane 4 : Human small intestine lysate
    Lane 5 : Mouse prostate lysate
    Lane 6 : Mouse kidney lysate
    Lane 7 : Rat colon lysate
    Lane 8 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3861] (ab92323)

    Unpurified ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-TMPRSS2 antibody [EPR3861] (ab92323)
    Anti-TMPRSS2 antibody [EPR3861] (ab92323)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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