Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: IDE knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109538 Anti-Insulin degrading enzyme / IDE antibody [EPR6099] was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261755 (knockout cell lysate ab257197) was used. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109538 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.