Anti-TMPRSS2 antibody (ab214462)
Key features and details
- Rabbit polyclonal to TMPRSS2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TMPRSS2 antibody
See all TMPRSS2 primary antibodies -
Description
Rabbit polyclonal to TMPRSS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human TMPRSS2 aa 320-370 conjugated to keyhole limpet haemocyanin.
Database link: O15393 -
Positive control
- WB: LnCaP, Caco 2 and Du145 cell lysates; Human prostate, lung, heart and brain tissues. IHC: Mouse lung tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMPRSS2 antibody (ab214462)All lanes : Anti-TMPRSS2 antibody (ab214462) at 1/500 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : Human prostate tissue lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Human lung tissue lysate
Lane 5 : Human heart tissue lysate
Lane 6 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab214462 observed at 52 kDa. Red - loading control, mouse anti GAPDH observed at 37kDa.
ab214462 was shown to react with TMPRSS2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab214462 and mouse anti GAPDH overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-TMPRSS2 antibody (ab214462)Anti-TMPRSS2 antibody (ab214462) at 1/300 dilution + Du145 cell lysate
Secondary
secondary antibody HRP-conjugated at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody (ab214462)Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse lung tissue labeling TMPRSS2 with ab214462 at 1/100 dilution, followed by conjugation to the secondary antibody and DAB staining.
