Anti-Stromal interaction molecule 1 antibody [5A2] (ab57834)
![Anti-Stromal interaction molecule 1 antibody [5A2] (ab57834)](https://www.abcam.com/ps/products/57/ab57834/Images/ab57834_1.jpg "Anti-Stromal interaction molecule 1 antibody [5A2] (ab57834)")
Key features and details
- Mouse monoclonal [5A2] to Stromal interaction molecule 1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Stromal interaction molecule 1 antibody [5A2]
See all Stromal interaction molecule 1 primary antibodies -
Description
Mouse monoclonal [5A2] to Stromal interaction molecule 1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein, corresponding to amino acids 24-686 of Human Stromal interaction molecule 1
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General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5A2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Stromal interaction molecule 1 antibody (ab57834)
Stromal interaction molecule 1 antibody (ab57834) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (ab57834)Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (ab57834)ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (ab57834) This image is courtesy of an anonymous Abreviewab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Stromal interaction molecule 1 antibody (ab57834)Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
