All lanes : Anti-Stromal interaction molecule 1 antibody (ab240592) at 0.4 µg/ml

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 77 kDa

Detection:  Chemiluminescence with exposure time of 3 minutes

 

Stromal interaction molecule 1 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab240592 at 6 μg/mL lysate. Western Blot was performed on the immunoprecipitate using ab240592 at 1 μg/mL.

Lane 1: ab240592 IP in HeLa whole cell lysate.
Lane 2: Control IgG.

Detection: Chemiluminescence with exposure times of 3 minutes