Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168)
Key features and details
- Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody
See all Muscarinic Acetylcholine Receptor M3/CHRM3 primary antibodies -
Description
Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human Muscarinic Acetylcholine Receptor M3/CHRM3 aa 286-461.
Database link: P20309 -
Positive control
- WB: NT2D1 whole cell lysate, mouse intestine tissue lysate. IF/ICC: A431 cells, SKNSH cells.
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General notes
This product was previously labelled as Muscarinic Acetylcholine Receptor M3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168)
Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling Muscarinic Acetylcholine Receptor M3/CHRM3 with ab126168 at 1/500 (green). Cells were fixed in ice-cold MeOH for 5 minutes. Cells were co-stained with Hoechst 33342 staining (blue). Blocking: 2.5% BSA/PBS for 30 min. The primary antibody was incubated for 4°C overnight. The secondary antibody was Rabbit IgG antibody (Alexa Fluor 488), 1/2000 (keep from light), room temperature for 1 hour. Washing using PBS 3 x 3 minutes.
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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168)Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168) at 1/1000 dilution + NT2D1 whole cell extracts at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 66 kDa7.5% SDS PAGE
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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168) This image is courtesy of an Abreview by Julien Chevalier.Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168) at 1/1000 dilution + Mouse intestine tissue lysate at 14 µg
Secondary
HRP-conjugated goat anti-Rabbit IgG (H+L) polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168)ICC/IF image of ab126168 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126158, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
