Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)
Key features and details
- Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody
See all Muscarinic Acetylcholine Receptor M3/CHRM3 primary antibodies -
Description
Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Pig, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Rat Muscarinic Acetylcholine Receptor M3/CHRM3 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab139964) -
Positive control
- This antibody gave a positive signal in the following Rat tissue lysates: Brain; Forebrain; Substantia Nigra; Heart.
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General notes
This product was previously labelled as Muscarinic Acetylcholine Receptor M3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab87199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 66 kDa). IHC-P Use a concentration of 5 µg/ml. Target
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Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281685 Cow
- Entrez Gene: 12671 Mouse
- Entrez Gene: 100144478 Pig
- Entrez Gene: 24260 Rat
- SwissProt: P41984 Cow
- SwissProt: Q9ERZ3 Mouse
- SwissProt: P11483 Pig
- SwissProt: P08483 Rat
see all -
Alternative names
- AChR antibody
- ACM3_HUMAN antibody
- cholinergic receptor muscarinic 3 antibody
see all
Images
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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)All lanes : Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain Rat Tissue Lysate
Lane 3 : Rat Substantia Nigra Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesMuscarinic Acetylcholine Receptor M3/CHRM3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)IHC image of Muscarinic Acetylcholine Receptor M3/CHRM3 staining in Rat normal brain (Sagittal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87199, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (6)
ab87199 has been referenced in 6 publications.
- Tarafder S et al. A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury. Theranostics 9:4241-4254 (2019). PubMed: 31281545
- Wu KC et al. I-Tiao-Gung extract through its active component daidzin improves cyclophosphamide-induced bladder dysfunction in rat model. Neurourol Urodyn 37:2560-2570 (2018). PubMed: 30252154
- Kashyap MP et al. BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity. Am J Physiol Renal Physiol 315:F45-F56 (2018). PubMed: 29092846
- Wilson C et al. Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium. FASEB J 30:2000-13 (2016). ICC . PubMed: 26873937
- Nishijima H et al. Denervation of nasal mucosa induced by posterior nasal neurectomy suppresses nasal secretion, not hypersensitivity, in an allergic rhinitis rat model. Lab Invest 96:981-93 (2016). PubMed: 27322954
- Eckhard A et al. Regulation of the perilymphatic-endolymphatic water shunt in the cochlea by membrane translocation of aquaporin-5. Pflugers Arch 467:2571-88 (2015). IF . PubMed: 26208470
Images
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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)All lanes : Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain Rat Tissue Lysate
Lane 3 : Rat Substantia Nigra Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesMuscarinic Acetylcholine Receptor M3/CHRM3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)
IHC image of Muscarinic Acetylcholine Receptor M3/CHRM3 staining in Rat normal brain (Sagittal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87199, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
