Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
![Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-320326-anti-stromal-interaction-molecule-1-antibody-epr3414-western-blot.jpg "Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Stromal interaction molecule 1 antibody [EPR3414]
See all Stromal interaction molecule 1 primary antibodies -
Description
Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3414 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108994 observed at 77 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. Ab108994 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-121528-anti-stromal-interaction-molecule-1-antibody-epr3414-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-121529-anti-stromal-interaction-molecule-1-antibody-epr3414-western-blot.jpg)
Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution
Lane 1 : SH SY5Y cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
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![Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-4-benefits-of-recombinant-antibodies.png)
Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
