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Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Stromal interaction molecule 1 antibody [EPR3414]
    See all Stromal interaction molecule 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 77 kDa
    Observed band size: 77 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab108994 observed at 77 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. Ab108994 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

    ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

    Lane 1 : SH SY5Y cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 77 kDa

  • Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
    Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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