Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARF6 antibody [EPR8357] (ab131261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8357] to ARF6
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARF6 antibody [EPR8357]
    See all ARF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR8357] to ARF6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, 293T, HEK-293, HAP1, HeLa and A431 cell lysates; Rat kidney lysates. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8357

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Anti-ARF6 antibody [EPR8357] (ab131261) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This antibody shows low affinity on HeLa cells and detects a strong non-specific band around 75 KD.

  • Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    All lanes : Anti-ARF6 antibody [EPR8357] (ab131261) at 1/200 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse kidney lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Anti-ARF6 antibody [EPR8357] (ab131261) at 1/1000 dilution (Purified) + Rat kidney lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ARF6 knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab131261 (unpurified) observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab131261 was shown to recognize ARF6 when ARF6 knockout samples were used, along with additional cross-reactive bands. Wild-type and ARF6 knockout samples were subjected to SDS-PAGE. ab131261 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    Western blot - Anti-ARF6 antibody [EPR8357] (ab131261)
    All lanes : Anti-ARF6 antibody [EPR8357] (ab131261) at 1/1000 dilution ((unpurified))

    Lane 1 : MCF7 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 20 kDa

  • Anti-ARF6 antibody [EPR8357] (ab131261)
    Anti-ARF6 antibody [EPR8357] (ab131261)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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