Anti-TRPC3 antibody (ab241343)
Key features and details
- Rabbit polyclonal to TRPC3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRPC3 antibody
See all TRPC3 primary antibodies -
Description
Rabbit polyclonal to TRPC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human TRPC3 aa 275-325. The exact sequence is proprietary.
Database link: Q13507 -
Positive control
- WB: HEK-392T and Jurkat cell lysate. IP: HEK-293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRPC3 antibody (ab241343)All lanes : Anti-TRPC3 antibody (ab241343) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate in NETN lysis buffer
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate in NETN lysis buffer
Lane 3 : HEK-293T cell lysate in RIPA lysis buffer
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate in NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-TRPC3 antibody (ab241343)TRPC3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241343 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241343 at 1 µg/ml.
Lane 1: ab241343 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN lysis buffer.
