Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280]
See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies
Description

Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

Rat

WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat cerebellum lysates; mouse cerebellum lysate (2%SDS treated, not boiled). IP: Mouse and rat cerebellum lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse Rat
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19280
Isotype

IgG
Research areas

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

Lane 1 : Mouse cerebellum lysate
Lane 2 : Rat cerebellum lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 102 kDa
Observed band size: ~300 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution + Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 102 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 102 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse cerebellum lysate, 10μg (Input).

Lane 2: ab184302 IP in Mouse cerebellum lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Rat cerebellum lysate, 10μg (Input).

Lane 2: ab184302 IP in Rat cerebellum lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com