Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)
![Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)](https://www.abcam.com/ps/products/16/ab16113/Images/ab16113-24844-anti-hair-cortex-cytokeratin-k40-antibody-ae13-western-blot.jpg "Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)")
Key features and details
- Mouse monoclonal [AE13] to hair cortex Cytokeratin/K40
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-hair cortex Cytokeratin/K40 antibody [AE13]
See all hair cortex Cytokeratin/K40 primary antibodies -
Description
Mouse monoclonal [AE13] to hair cortex Cytokeratin/K40 -
Host species
Mouse -
Specificity
This antibody is specific for the family of hair cortex keratins. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Full length protein corresponding to Human hair cortex Cytokeratin/K40.
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General notes
This antibody is an excellent marker for hair and nail differentiation.
Previously labelled as hair cortex Cytokeratin.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody is an excellent marker for hair and nail differentiation. -
Clonality
Monoclonal -
Clone number
AE13 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)All lanes : Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113) at 1 µg/ml
Lane 1 :Mouse skin tissue lysate (14 days) (ab7271)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 - 46 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)](https://www.abcam.com/ps/products/16/ab16113/Images/ab16113-24843-anti-hair-cortex-cytokeratin-k40-antibody-ae13-flow-cytometry.jpg)
Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
