Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18783] to CIRP - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CIRP antibody [EPR18783] - BSA and Azide free
    See all CIRP primary antibodies

  • Description

    Rabbit monoclonal [EPR18783] to CIRP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T cell lysate. IP: HeLa cells. IHC-P: Human breast cancer and endometrium tissue. ICC/IF: HeLa and HCT 116 cells.

  • General notes

    Ab238946 is the carrier-free version of ab191885. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Western blot - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    All lanes : Anti-CIRP antibody [EPR18783] (ab191885) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : CIRP knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 19 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab191885).

    Lanes 1-2: Merged signal (red and green). Green - ab191885 observed at 19 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab191885 Anti-CIRP antibody [EPR18783] was shown to specifically react with CIRP in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266187 (knockout cell lysate ab257252) was used. Wild-type and CIRP knockout samples were subjected to SDS-PAGE. ab191885 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling CIRP using ab191885 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab191885, and secondary antibody only.
    Note: Nuclear and weak cytoplasm staining on tumor cells of breast cancer.
    Mol Carcinog. 2010 49, 130–140.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191885).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Immunocytochemistry/ Immunofluorescence - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CIRP with ab191885 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab191885 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191885).

  • Immunocytochemistry/ Immunofluorescence - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Immunocytochemistry/ Immunofluorescence - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling CIRP with ab191885 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HCT 116 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab191885 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191885).

  • Immunoprecipitation - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Immunoprecipitation - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

    Immunoprecipitation of CIRP from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate achieved using ab191885 at 1/20 dilution.
    Lane 1: Input: 10µg of HeLa whole cell lysate.
    Lane 2: HeLa whole cell lysate following IP with ab191885.
    Lane 3: negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab191885 in HeLa whole cell lysates.
    Western blot was performed using ab191885 at 1/1000 dilution.
    VeriBlot for IP secondary antibody (HRP) (ab131366) was used for detection at 1/10000 dilution.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 3 second exposure.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191885).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling CIRP using ab191885 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab191885, and secondary antibody only.
    Note: Nuclear staining on normal human endometrium.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191885).

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)
    Anti-CIRP antibody [EPR18783] - BSA and Azide free (ab238946)

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