Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21122] to BCRP/ABCG2 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free
    See all BCRP/ABCG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21122] to BCRP/ABCG2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human placenta tissue.

  • General notes

    Ab230160 is the carrier-free version of ab229193. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230160 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Flow Cytometry - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

    Flow cytometric analysis of A549 (human lung carcinoma cell line) cell line labeling BCRP/ABCG2 with ab229193 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on total viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229193).

  • Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized JAR (human placenta choriocarcinoma cell line) cells labeling BCRP/ABCG2 with ab229193 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in JAR cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229193).

  • Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling BCRP/ABCG2 with ab229193 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in A549 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229193).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

    Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue labeling BCRP/ABCG2 with ab229193 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) . Membrane and cytoplasmic staining in esophageal cancer (PMID: 17671398) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229193).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

    Immunohistochemical analysis of paraffin-embedded human brain tissue labeling BCRP/ABCG2 with ab229193 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in endothelial cells of human brain (PMID: 28912477) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229193).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling BCRP/ABCG2 with ab229193 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human placenta (PMID: 28623970) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229193).

  • Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)
    Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free (ab230160)

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