Anti-BCRP/ABCG2 antibody [BXP-21] (ab3380)
Key features and details
- Mouse monoclonal [BXP-21] to BCRP/ABCG2
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-BCRP/ABCG2 antibody [BXP-21]
See all BCRP/ABCG2 primary antibodies -
Description
Mouse monoclonal [BXP-21] to BCRP/ABCG2 -
Host species
Mouse -
Specificity
BXP-21 reacts with an internal epitope of BCRP/ABCG2, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. This antibody does not react with the human MDR1, MRP1 and MRP2 gene products.
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Species reactivity
Reacts with: Human -
Immunogen
Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP, GeneBank accession #AF098951].
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Positive control
- IHC-P: Human breast cancer tissue. ICC/IF: MCF7 cells. Caco Cell lysate (see second reference cited).
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General notes
For the WB, please note the subcellular localization and distribution of the BCRP/ABCG2 in Normal Human Tissues: PMID:11309308
We have received mixed feedback from customers, the antibody showed 45-50 kDa band size in A549 and Caco2 cell lines against predicted size of 72kDa. In response to data we have received we no longer guarantee this antibody for western blot. ABCG2 is a multi-pass membrane protein so for troubleshooting we would recommend heating the samples at 60-70C for 15-20 minutes instead of boiling and as this protein exists as homodimer so complete reduction of samples is highly recommended. In case of any other question please email scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: 0.7% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone. -
Clonality
Monoclonal -
Clone number
BXP-21 -
Isotype
IgG2a -
Research areas
