Recombinant mouse IGF1 protein (Active) (ab280348)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
Preparation and Storage
- IBP1
- IGF I
- IGF IA
Images
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Functional Studies - Recombinant mouse IGF1 protein (Active) (ab280348)
Fully biologically active determined by the dose dependent increase in MCF7 cell proliferation.
ED50 is ≤47.5 ng/mL, corresponding to a specifc activity of 0.021 x 106 units/mg.
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SDS-PAGE - Recombinant mouse IGF1 protein (Active) (ab280348)SDS-PAGE analysis of ab280348.
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Mass Spectrometry - Recombinant mouse IGF1 protein (Active) (ab280348)Mass determination by ESI-TOF.
Predicted MW is 15077.3 Da (+/- 10 Da by ESI-TOF). Observed MW is 15077.37. Multiple O-glycosylation sites are present in the sequence and presented in the mass analysis.
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HPLC - Recombinant mouse IGF1 protein (Active) (ab280348)HPLC analysis of ab280348.