Human Complement C4-Binding Protein ELISA Kit (ab222866)
Key features and details
- Sensitivity: 0.9 ng/ml
- Range: 0.938 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C4-Binding Protein ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 7.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.9 ng/ml -
Range
0.938 ng/ml - 60 ng/ml -
Recovery
97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow, Dog, Pig, Monkey -
Product overview
ab222866 Human Complement C4-Binding Protein ELISA Kit is designed for the quantitative measurement of Complement C4-Binding Protein in plasma, serum, urine, milk, saliva, cerebrospinal fluid (CSF), and cell culture samples.
The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Complement C4-Binding Protein (C4BP) in less than 4 hours. A polyclonal antibody specific for human complement C4BP has been pre-coated onto a 96-well microplate with removable strips. Complement C4BP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for complement C4BP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Complement component C4-binding protein (C4BP) regulates the complement system by accelerating the decay of the complement component C3 convertase and by acting as a cofactor to the serine protease factor I in the degradation of C4b. C4BP is a high molecular mass (570 kDa) glycoprotein and is present in plasma in various isoforms with different alpha beta composition. The major form of C4BP is composed of seven identical 70-kDa alpha chains, each containing a binding site for the complement protein C4b, and a unique 45 kDa beta chain which contains a binding site for the vitamin K-dependent protein S. C4BP was overexpressed in the synovial membranes of patients with rheumatoid arthritis. It was detected in amyloid-beta plaques and on apoptotic cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C4BP 1 x 120µl Anti- Human Complement C4BP coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 7ml Human Complement C4BP Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S. -
Sequence similarities
Contains 3 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C4b binding protein beta chain
- C4b-binding protein beta chain
- C4BP
see all -
Database links
- Entrez Gene: 725 Human
- Omim: 120831 Human
- SwissProt: P20851 Human
- Unigene: 99886 Human
Images
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Human Complement C4-Binding Protein ELISA Kit (ab222866)
Example of human Complement C4-Binding Protein standard curve.