Anandamide (ethanol solution), endogenous cannabinoid (ab120087)
Key features and details
- Endogenous cannabinoid
- CAS Number: 94421-68-8
- Purity: > 98%
- Supplied in ethanol (5 mg/ml)
- Form / State: Liquid
- Source: Synthetic
Overview
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Product name
Anandamide (ethanol solution), endogenous cannabinoid -
Description
Endogenous cannabinoid -
Alternative names
- AEA
- Arachidonylethanolamide
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Biological description
Endogenous cannabinoid, binding to CB1, CB2 and vanilloid receptors. Cannabimimetic in vivo.
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Purity
> 98% -
CAS Number
94421-68-8 -
Chemical structure
Properties
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Chemical name
(5Z,8Z,11Z,14Z)-N-(2-Hydroxyethyl)icosa-5,8,11,14-tetraenamide -
Molecular weight
347.54 -
Molecular formula
C22H37NO2 -
PubChem identifier
5281969 -
Storage instructions
Store at -20°C (desiccating conditions). -
Solubility overview
Supplied in ethanol (5 mg/ml) -
Handling
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(CCC/C=CC/C=CC/C=CC/C=CCCCCC)NCCO -
Source
Synthetic
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Research areas
Images
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Functional Studies - Anandamide (ethanol solution), endogenous cannabinoid (ab120087)ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) (ab120087), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120087 (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.
