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Epigenetics and Nuclear Signaling DNA methylation DNMTs

DNMT3B Assay Kit (ab113471)

DNMT3B Assay Kit (ab113471)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Platform: Microplate reader
  • Assay time: 3 hr

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Overview

  • Product name

    DNMT3B Assay Kit
  • Assay time

    3h 00m
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    DNMT3B (DNA (cytosine-5)-methyltransferase 3B) belongs to the DNMT family, it is required for genome de novo methylation and it is essential for establishment of DNA methylation patterns during development.

    DNMT3B Assay Kit allows the user to quickly and consistently measure the amount of DNMT3B. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B assay experiment. DNMT3B Assay Kit is suitable for quantitative measurement of DNMT3B amounts from fresh tissue and cultured cells of human and mouse.

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 48 tests 96 tests
    10X Wash Buffer 1 x 12ml 1 x 25ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Assay Buffer 1 x 5ml 1 x 10ml
    Blocking Buffer 1 x 10ml 1 x 20ml
    Capture Antibody, 100 µg/mL 1 x 8µl 1 x 16µl
    Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl
    Developing Solution 1 x 6ml 1 x 12ml
    DNMT3B Standard, 20 µg/mL 1 x 16µl 1 x 30µl
    Stop Solution 1 x 6ml 1 x 11ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • DNA modification and methylation

Images

  • Functional Studies - DNMT3B Assay Kit (ab113471)
    Functional Studies - DNMT3B Assay Kit (ab113471)

    Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and DNMT3B activity was measured using ab113471. DNMT1 and DNMT3A activities were measured using ab113469 DNMT1 Assay Kit and ab113470 DNMT3A Assay Kit.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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