Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3042] to Rab4 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rab4 antibody [EPR3042] - BSA and Azide free
    See all Rab4 primary antibodies

  • Description

    Rabbit monoclonal [EPR3042] to Rab4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate.

  • General notes

    Ab247727 is the carrier-free version of ab108974. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247727 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)
    Western blot - Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)
    All lanes : Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RAB4A knockout HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 24 kDa



    This data was developed using the same antibody clone in a different buffer formation (ab108974).

    Lanes 1- 2: Merged signal (red and green). Green - ab108974 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab108974 was shown to react with Rab4 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264781 (knockout cell lysate ab257624) was used. Wild-type HeLa and RAB4A knockout HeLa cell lysates were subjected to SDS-PAGE. ab108974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)
    Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.