Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)
![Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)](https://www.abcam.com/ps/products/247/ab247727/Images/ab247727-393536-anti-rab4-antibody-epr3042-early-endosome-marker-western-blot-hela-lysates.jpg "Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3042] to Rab4 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Rab4 antibody [EPR3042] - BSA and Azide free
See all Rab4 primary antibodies -
Description
Rabbit monoclonal [EPR3042] to Rab4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate.
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General notes
Ab247727 is the carrier-free version of ab108974. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247727 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3042 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)All lanes : Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RAB4A knockout HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDaThis data was developed using the same antibody clone in a different buffer formation (ab108974).
Lanes 1- 2: Merged signal (red and green). Green - ab108974 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108974 was shown to react with Rab4 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264781 (knockout cell lysate ab257624) was used. Wild-type HeLa and RAB4A knockout HeLa cell lysates were subjected to SDS-PAGE. ab108974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)](https://www.abcam.com/ps/products/247/ab247727/Images/ab247727-2-benefits-of-recombinant-antibodies.png)
Anti-Rab4 antibody [EPR3042] - BSA and Azide free (ab247727)
