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Human CANX (Calnexin) knockout HEK293T cell pellet (ab278994)

Overview

  • Product name

    Human CANX (Calnexin) knockout HEK293T cell pellet
    See all Calnexin kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

  • Sequence similarities

    Belongs to the calreticulin family.

  • Cellular localization

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Alternative names

    • Calnexin
    • CALX_HUMAN
    • CANX
    • CNX
    • FLJ26570
    • Histocompatibility complex class I antigen binding protein p88
    • IP90
    • Major histocompatibility complex class I antigen-binding protein p88
    • p90
    see all

Properties

Images

  • Western blot - Human CANX (Calnexin) knockout HEK293T cell pellet (ab278994)
    Western blot - Human CANX (Calnexin) knockout HEK293T cell pellet (ab278994)
    Lane 1: Wild-type HEK-293T cell lysate (20µg)

    Lane 2: CANX knockout HEK-293T cell lysate (20µg)
    Lanes 1- 2: Merged signal (red and green). Green - ab133615 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.
    ab133615 Recombinant Anti-Calnexin antibody [EPR3632] was shown to specifically react with CANX in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255368 (knockout cell lysate ab263805) was used. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133615 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human CANX (Calnexin) knockout HEK293T cell pellet (ab278994)
    Sanger Sequencing - Human CANX (Calnexin) knockout HEK293T cell pellet (ab278994)
    Homozygous: 19 bp deletion in exon2

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