Anti-TRAF6 antibody (ab137452)
Key features and details
- Rabbit polyclonal to TRAF6
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRAF6 antibody
See all TRAF6 primary antibodies -
Description
Rabbit polyclonal to TRAF6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to a region within amino acids 144-408 of Human TRAF6 (UniProt ID: Q9Y4K3).
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Positive control
- Purchase matching WB positive control:Recombinant Human TRAF6 protein
- WB: A431, A549, HeLa, HepG2,NIH/3T3, BCL-1, PC-12, Rat2 cell lysate. IHC-P: 59T xenograft, mouse lung and rat brain tissue. IF: HeLa cells. IP: K562 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRAF6 antibody (ab137452)Anti-TRAF6 antibody (ab137452) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 60 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (ab137452)Paraffin-embedded 59T xenograft tissue stained TRAF6 using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (ab137452)Immunofluorescence analysis of 4% paraformaldehyde-fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TRAF6 at cytoplasm and nucleus (green) with ab137452 at 1/100 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-TRAF6 antibody (ab137452)IP samples: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate.
1- Control with 4 μg of preimmune Rabbit IgG;
2- Immunoprecipitation of TRAF6 protein by 4 μg ab137452.7.5 % SDS-PAGE
The immunoprecipitated TRAF6 protein was detected by ab137452 at 1/500 dilution, followed by anti-rabbit IgG antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (ab137452)Paraffin-embedded mouse lung tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Western blot - Anti-TRAF6 antibody (ab137452)All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (ab137452)Paraffin-embedded rat brain tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Western blot - Anti-TRAF6 antibody (ab137452)All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa7.5% SDS PAGE
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Western blot - Anti-TRAF6 antibody (ab137452)All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa7.5% SDS-PAGE
