Anti-SHARPIN antibody (ab125188)
Key features and details
- Rabbit polyclonal to SHARPIN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHARPIN antibody
See all SHARPIN primary antibodies -
Description
Rabbit polyclonal to SHARPIN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human SHARPIN (NP_112236.3).
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Positive control
- HeLa, Jurkat and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHARPIN antibody (ab125188)All lanes : Anti-SHARPIN antibody (ab125188) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-SHARPIN antibody (ab125188)ab125188, at 6 µg/mg lysate, detecting SHARPIN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125188 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 30 seconds.
