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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins

Anti-SHARPIN antibody (ab197853)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-SHARPIN antibody (ab197853)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SHARPIN
  • Suitable for: IP, WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SHARPIN antibody
    See all SHARPIN primary antibodies
  • Description

    Rabbit polyclonal to SHARPIN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human SHARPIN aa 1-250 (N terminal).
    Database link: Q9H0F6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Sharpin transfected U2OS cells and lysates, U2OS cell extracts, NIH3T3 cell lysate,
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins

Images

  • Western blot - Anti-SHARPIN antibody (ab197853)
    Western blot - Anti-SHARPIN antibody (ab197853)
    All lanes : Anti-SHARPIN antibody (ab197853) at 1/2000 dilution

    Lane 1 : Control siRNA transfected U2OS cell lysate
    Lane 2 : Sharpin siRNA transfected U2OS cell lysate
    Lane 3 : NIH3T3 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 40 kDa



    Transfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ 4oC in 5% milk TBST (0.1% Tween).

  • Immunoprecipitation - Anti-SHARPIN antibody (ab197853)
    Immunoprecipitation - Anti-SHARPIN antibody (ab197853)

    Western blot analysis with ab197853 of Immunoprecipitation
    using U2OS cells extracts
    Lane 1: Input.
    Lane 2: Immunoprecipitation with control serum.
    Lane 3: Immunoprecipitation with ab197853 (5μl)

  • Flow Cytometry - Anti-SHARPIN antibody (ab197853)
    Flow Cytometry - Anti-SHARPIN antibody (ab197853)

    Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells.  Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.

  • Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (ab197853)
    Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (ab197853)

    Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IP, WB

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