Anti-SHARPIN antibody (ab197853)
Key features and details
- Rabbit polyclonal to SHARPIN
- Suitable for: IP, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SHARPIN antibody
See all SHARPIN primary antibodies -
Description
Rabbit polyclonal to SHARPIN -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human SHARPIN aa 1-250 (N terminal).
Database link: Q9H0F6 -
Positive control
- Sharpin transfected U2OS cells and lysates, U2OS cell extracts, NIH3T3 cell lysate,
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SHARPIN antibody (ab197853) at 1/2000 dilution
Lane 1 : Control siRNA transfected U2OS cell lysate
Lane 2 : Sharpin siRNA transfected U2OS cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 40 kDaTransfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ 4oC in 5% milk TBST (0.1% Tween).
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Western blot analysis with ab197853 of Immunoprecipitation
using U2OS cells extracts
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab197853 (5μl) -
Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.
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Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)