Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant HSP65 protein (ab92416)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: WB, Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant HSP65 protein
    See all HSP65 proteins and peptides

  • Biological activity

    This product does not contain GroEL.

  • Purity

    > 90 % SDS-PAGE.
    ab92416 is purified by Multi-step chromatography

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mycobacterium bovis

    • Specifications

    Our Abpromise guarantee covers the use of ab92416 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      Functional Studies

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      This product does not contain GroEL.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      Constituents: 0.01999% Monobasic dihydrogen potassium phosphate, 0.11445% Dibasic monohydrogen sodium phosphate, 0.02025% Potassium chloride, 0.8004% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Relevance

      HSP65 is a member of the HSP60 family of heat shock proteins isolated from Mycobacterium bovis BCG. HSP65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. HSP65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab92416? Please let us know so that we can cite the reference in this datasheet.

ab92416 has been referenced in 1 publication.

  • Kim BJ  et al. Development of a Live Recombinant BCG Expressing Human Immunodeficiency Virus Type 1 (HIV-1) Gag Using a pMyong2 Vector System: Potential Use As a Novel HIV-1 Vaccine. Front Immunol 9:643 (2018). PubMed: 29636755

Preparation and Storage

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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