Anti-SHARPIN antibody (ab227598)
Key features and details
- Rabbit polyclonal to SHARPIN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHARPIN antibody
See all SHARPIN primary antibodies -
Description
Rabbit polyclonal to SHARPIN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human SHARPIN (internal sequence). The exact sequence is proprietary.
Database link: Q9H0F6 -
Positive control
- IP: THP-1 whole cell extract. WB: K562 and THP-1 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SHARPIN antibody (ab227598)
SHARPIN was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extracts using 5 μg ab227598. Western blot analysis was performed using ab227598. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in THP-1 whole cell extract.
Lane 2: ab227598 in THP-1 whole cell extract.
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Western blot - Anti-SHARPIN antibody (ab227598)All lanes : Anti-SHARPIN antibody (ab227598) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Predicted band size: 40 kDa10% SDS-PAGE gel.
