Anti-EMILIN1 antibody (ab125860)
Key features and details
- Rabbit polyclonal to EMILIN1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EMILIN1 antibody
See all EMILIN1 primary antibodies -
Description
Rabbit polyclonal to EMILIN1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human EMILIN1 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab156174) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Small Intestine; Human Cervix; Human Fetal Heart; Human Fetal Kidney.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RabbitCowDogPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB 1/250. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).Notes WB
1/250. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).Target
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Function
May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. -
Tissue specificity
Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain.
Contains 1 EMI domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils. - Information by UniProt
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Database links
- Entrez Gene: 11117 Human
- Omim: 130660 Human
- SwissProt: Q9Y6C2 Human
- Unigene: 63348 Human
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Alternative names
- Elastin microfibril interface located protein 1 antibody
- Elastin microfibril interface located protein antibody
- Elastin microfibril interface-located protein 1 antibody
see all
Images
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Western blot - Anti-EMILIN1 antibody (ab125860)All lanes : Anti-EMILIN1 antibody (ab125860) at 1/250 dilution
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : Human cervix normal tissue lysate - total protein (ab29226)
Lane 3 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lane 4 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
EMILIN1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125860 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab125860 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-EMILIN1 antibody (ab125860)All lanes : Anti-EMILIN1 antibody (ab125860) at 1/250 dilution
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : Human cervix normal tissue lysate - total protein (ab29226)
Lane 3 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lane 4 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
EMILIN1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125860 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
