Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)
Key features and details
- Rabbit polyclonal to Metabotropic Glutamate Receptor 4/MGLUR4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Cat, Human
- Isotype: IgG
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody
See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies -
Description
Rabbit polyclonal to Metabotropic Glutamate Receptor 4/MGLUR4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Cat, Human -
Immunogen
Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 4/MGLUR4 (C terminal).
Database link: Q14833 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)Ab53088 staining Human cerebellum. Staining is localised to the cell membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088) at 1/500 dilution
Lane 1 : 293 cell extracts, treated
with Forskolin (40nM, 30mins)
Lane 2 : 293 cell extracts, treated
with Forskolin (40nM, 30mins) with peptide
Predicted band size: 102 kDa
Observed band size: 102 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)ICC/IF image of ab53088 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53088, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
