Anti-EAAT1 antibody (ab416)
Key features and details
- Rabbit polyclonal to EAAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EAAT1 antibody
See all EAAT1 primary antibodies -
Description
Rabbit polyclonal to EAAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
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General notes
Glutamate-aspartate transporter is also known as GLAST.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EAAT1 antibody (ab416)Anti-EAAT1 antibody (ab416) + Rat brain cortex
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 150 kDa (possible dimer)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
