Recombinant human GAD65 protein (ab206646)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
-
Product name
Recombinant human GAD65 protein
See all GAD65 proteins and peptides -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPA CDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTA NTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYA MMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAV ADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGV PLQCSALLVREEGLMQNCNQ MHASYLFQQDKHYDLSYDTGDKALQCGR HVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGK PQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQ PLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL -
Predicted molecular weight
65 kDa -
Amino acids
1 to 585 -
Additional sequence information
Recombinant human GAD65 expressed in baculoviral infection of Spodoptera frugiperda cells, purified & formulated in RPMI medium in human serum albumin (clinical grade) for use in T cell assays.
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: Human serum albumin, 0.03% DTT
RPMI bufferThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
SDS-PAGE - Recombinant human GAD65 protein (ab206646)
SDS Page Gel analysis comparing different protein molecular weights, lanes 1 & 2 contain rhGAD65 ab206647 at concentrations of 1 µL & 0.1 µL respectively. This protein was compared to BSA (lane 3 - 1 µg & lane 4 - 10 µg) and T-cell GAD65 (ab206646) (lane 5 - 1 µL & lane 6 - 0.1 µL). The bands were run on a NuPAGE 7% Tris-Acetate Gel.
