Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)
![Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)](https://www.abcam.com/ps/products/251/ab251442/Images/ab251442-410582-anti-pro-egf-antibody-epr14622-bsa-and-azide-free-western-blot.jpg "Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14622] to pro-EGF - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-pro-EGF antibody [EPR14622] - BSA and Azide free
See all pro-EGF primary antibodies -
Description
Rabbit monoclonal [EPR14622] to pro-EGF - BSA and Azide free -
Host species
Rabbit -
Specificity
Recognises the Pro-epidermal growth factor chain.
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Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251442 is the carrier-free version of ab206106. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251442 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14622 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)All lanes : Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution
Lane 1 : Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag
Lane 2 : Recombinant Human EGF recombinant protein (aa971-1023)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 134 kDa
Exposure time: 1 secondThis data was developed using ab206106, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)](https://www.abcam.com/ps/products/251/ab251442/Images/ab251442-408283-anti-pro-egf-antibody-epr14622-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/5000 dilution + Human EGF recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 134 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab206106, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human EGF full length protein contains aa21-1023 with His-Tag® at the C-Terminus.
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![Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)](https://www.abcam.com/ps/products/251/ab251442/Images/ab251442-406058-anti-pro-egf-antibody-epr14622-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution + Human milk sample at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 134 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab206106, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)](https://www.abcam.com/ps/products/251/ab251442/Images/ab251442-403400-anti-pro-egf-antibody-epr14622-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution + Human urine sample at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 134 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab206106, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)](https://www.abcam.com/ps/products/251/ab251442/Images/ab251442-1-anti-pro-egf-antibody-epr14622-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)This data was developed using ab206106, the same antibody clone in a different buffer formulation.EGF was immunoprecipitated from 1mg of Human milk sample with ab206106 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206106 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Human milk sample 10ug (Input). Lane 2: ab206106 IP in Human milk sample. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206106 in Human milk sample. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes. -
![Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)](https://www.abcam.com/ps/products/251/ab251442/Images/ab251442-6-benefits-of-recombinant-antibodies.png)
Anti-pro-EGF antibody [EPR14622] - BSA and Azide free (ab251442)