Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling S100 beta with ab218516 at 2 µg/mL (30 minutes at room temperature). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.

Anti-S100 beta antibody [SH-B1] (ab218516) at 2 µg/ml + Human brain tissue lysate

Predicted band size: 10 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labeling S100 beta with ab218516 at 2 µg/mL (30 minutes at room temperature). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.

ab218516 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.