Anti-Daxx (phospho S668) antibody (ab55323)
Key features and details
- Rabbit polyclonal to Daxx (phospho S668)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Daxx (phospho S668) antibody
See all Daxx primary antibodies -
Description
Rabbit polyclonal to Daxx (phospho S668) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Daxx only when phosphorylated at serine 668. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic phosphopeptide derived from human Daxx around the phosphorylation site of serine 668 (L-P-SP-P-P).
-
Positive control
- Extracts form 293 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Daxx (phospho S668) antibody (ab55323)All lanes : Anti-Daxx (phospho S668) antibody (ab55323) at 1/500 dilution
Lane 1 : Extracts from 293 cells treated with PBS for 60 minutes.
Lane 2 : Extracts from 293 cells treated with PBS for 60 minutes, and with the immunising phosphopeptide.
Predicted band size: 82 kDa
Observed band size: 82 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Daxx (phospho S668) antibody (ab55323)Ab55323 staining human normal placenta tissue. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requ
