TACE Inhibitor Assay Kit (ab155889)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
Overview
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Product name
TACE Inhibitor Assay Kit -
Detection method
Fluorescent -
Assay type
Quantitative -
Assay time
0h 20m -
Product overview
In Abcam’s TACE inhibitor screening Kit, TACE (tumor necrosis factor-α-converting enzyme) hydrolyzes the specific FRET substrate to release the quenched fluorescent group, which can be detected fluorometrically at Ex/Em = 318/449 nm. In the presence of the potent TACE inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor Control GM6001 is included to compare the efficacy of test inhibitors.
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Notes
The TACE (tumor necrosis factor-α-converting enzyme), also called ADAM metallopeptidase domain 17 (ADAM17), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. TACE is believed to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests TACE Assay Buffer WM 1 x 25ml TACE Enzyme Green 1 vial TACE Inhibitor Control Purple 1 x 20µl TACE Substrate Red 1 x 0.2ml -
Research areas
Images
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GM6001 Inhibition of TACE Activity
TACE activity with increasing GM6001 inhibition. Produced following kit protocol.
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TACE Enzyme Control and BackgroundFluorescence of TACE enzyme control and background. Produced following kit protocol.