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Anti-FSH beta antibody [EPR24439-141] (ab281562)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24439-141] to FSH beta
Suitable for: IHC-P, IHC-Fr, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-FSH beta antibody [EPR24439-141]
See all FSH beta primary antibodies
Description

Rabbit monoclonal [EPR24439-141] to FSH beta
Host species

Rabbit
Tested applications

Suitable for: IHC-P, IHC-Fr, WBmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human pituitary, Mouse pituitary, Rat pituitary lysates. IHC-P: Human pituitary, Mouse pituitary, Rat pituitary tissues. IHC-Fr: Rat pituitary tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24439-141
Isotype

IgG

Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)

All lanes : Anti-FSH beta antibody [EPR24439-141] (ab281562) at 1/1000 dilution

Lane 1 : Human pituitary tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Human spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 15 kDa
Observed band size: 23,19 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID: 29090093, 11502795).

Negative control: heart, spleen (PMID:29090093).

Exposure time: 81 seconds
Western blot - Anti-FSH beta antibody [EPR24439-141] (ab281562)

All lanes : Anti-FSH beta antibody [EPR24439-141] (ab281562) at 1/1000 dilution

Lane 1 : Mouse pituitary tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat pituitary tissue lysate
Lane 5 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 15 kDa
Observed band size: 23,19 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID: 29090093, 11502795).

Negative control: heart, spleen (PMID:29090093).

Exposure time: Lanes 1-3:3 minutes; Lanes 4-5:26 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)

Immunohistochemical analysis of paraffin-embedded Human pituitary tissue labelling FSH beta with ab281562 at 1/2000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pituitary (PMID: 8930609). The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)

Immunohistochemical analysis of paraffin-embedded Mouse pituitary tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pituitary. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)

Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat pituitary. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pituitary tissue labeling FSH beta with ab281562 at 1/100 dilution (4.75 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat pituitary is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FSH beta antibody [EPR24439-141] (ab281562)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling FSH beta with ab281562 at 1/4000 dilution (0.119 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cardiac muscle. The section was incubated with ab281562 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-FSH beta antibody [EPR24439-141] (ab281562)

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