Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19669] to Integrin alpha V - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free
    See all Integrin alpha V primary antibodies

  • Description

    Rabbit monoclonal [EPR19669] to Integrin alpha V - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251483 is the carrier-free version of ab208012. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    Western blot - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    All lanes : Anti-Integrin alpha V antibody [EPR19669] (ab208012) at 1/2000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 87 kDa
    Observed band size: 130-140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab208012, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22740495).

  • Western blot - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    Western blot - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    All lanes : Anti-Integrin alpha V antibody [EPR19669] (ab208012) at 1/2000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 87 kDa
    Observed band size: 130-140 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab208012, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    Western blot - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    All lanes : Anti-Integrin alpha V antibody [EPR19669] (ab208012) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Mouse kidney tissue lysate
    Lane 7 : Mouse spleen tissue lysate
    Lane 8 : Rat kidney tissue lysate
    Lane 9 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 µg

    Predicted band size: 87 kDa
    Observed band size: 130-140 kDa why is the actual band size different from the predicted?



    This data was developed using ab208012, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: Lanes 1-3: 5 seconds; Lanes 4 and 9: 30 seconds; Lanes 5-7: 1 minute; Lane 8: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    This data was developed using ab208012, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD51 with ab208012 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and membrane staining on human kidney is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    This data was developed using ab208012, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CD51 with ab208012 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on human bladder cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)
    Anti-Integrin alpha V antibody [EPR19669] - BSA and Azide free (ab251483)

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