HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab202580)
![HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab202580)](https://www.abcam.com/ps/products/202/ab202580/Images/ab202580-246444-anti-crispr-cas9-antibody-7a9-3a3-hrp-western-blot.jpg "HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab202580)")
Key features and details
- HRP Mouse monoclonal [7A9-3A3] to CRISPR-Cas9
- Suitable for: WB
- Reacts with: Streptococcus pyogenes
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-CRISPR-Cas9 antibody [7A9-3A3]
See all CRISPR-Cas9 primary antibodies -
Description
HRP Mouse monoclonal [7A9-3A3] to CRISPR-Cas9 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Streptococcus pyogenes -
Immunogen
Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (N terminal).
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Positive control
- WB: S2 cells transfected with CRISPR-Cas9
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
7A9-3A3 -
Isotype
IgG1 -
Light chain type
kappa
Images
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Western blot - HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab202580)All lanes : HRP Anti-CRISPR-Cas9 antibody [7A9-3A3] (ab202580) at 1/5000 dilution
Lane 1 : Cas 9 negative control (S2 non-transfected cell lysate)
Lane 2 : Cas 9 transfected cells (S2 cells transfected with CRISPR-Cas9 lysate)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202580 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
