Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
![Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.com/ps/products/177/ab177483/Images/ab177483-341537-ab177483.jpg "Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
See all GABA Transporter 1 / GAT 1 primary antibodies -
Description
Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P30531 -
Positive control
- Fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12998 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1 : Human brain lysates unboiled
Lane 2 : Human brain lysates boiled
Lane 3 : Mouse brain unboiled
Lane 4 : Mouse brain boiled
Lane 5 : Rat brain unboiled
Lane 6 : Rat brain boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 2: 20 seconds
Lane 3 to 6: 8 secondsWe recommend not to boil the samples after lysis to get desired WB results.
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![Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.com/ps/products/177/ab177483/Images/ab177483-205382-anti-gaba-transporter-1-gat-1-antibody-epr12998-western-blot.jpg)
Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg
Predicted band size: 67 kDa
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![Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)](https://www.abcam.com/ps/products/177/ab177483/Images/ab177483-3-benefits-of-recombinant-antibodies.png)
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)
