Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2 - BSA and Azide free
  • Suitable for: ICC, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free
    See all LRRK2 primary antibodies

  • Description

    Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human LRRK2 aa 950 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: MEF and A549 cell lysates ICC/IF: Neuro-2a, and SH-SY5Y-DRD1 cells IHC: Mouse brain tissue

  • General notes

    ab172378 is the carrier-free version of ab133474 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab172378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
    Western blot - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

    This WB data was generated using the same anti-LRRK2 antibody clone, MJFF2 (c41-2), in a different buffer formulation (cat# ab133474).

    Lane 1: Wild type A549 whole cell lysate (20 µg)
    Lane 2: Wild type MEF whole cell lysate (20 µg)
    Lane 3: LRRK2 knockout A549 whole cell lysate (20 µg)
    Lane 4: LRRK2 knockout MEF whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133474 observed at 286 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab133474 was shown to recognize LRRK2 in wild type A549 and MEF cells along with additional cross reative bands. Whilst signal was not seen in LRRK2 knockout cells. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. Ab133474 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    Wild-type and LRRK2 knockout MEF and A549 cells were provide as a generous gift from Professor Dario Alessi, MRC Protein Phosphorylation and Ubiquitination Unit (University of Dundee).

  • Immunocytochemistry - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
    Immunocytochemistry - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

    ab133474 staining LRRK2 in Neuro-2a (mouse neuroblastoma cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). ab150077 was used as the secondary antibody (1/1000). Tubulin stained using ab7291 (1/1000) and ab150120 (1/1000) as the secondary. Nuclear counter stained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133474).

  • Immunocytochemistry - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
    Immunocytochemistry - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378) Rassu M. et al. PLoS One. 2017 Jun 5;12(6):e0179082. doi: 10.1371/journal.pone.0179082. eCollection 2017.

    Evaluation of DRD1 intracellular and extracellular level by BPA upon dopamine treatment in SH-SY5Y-DRD1 cells untransduced or transduced by WT or G2019S LRRK2

    (C and D) DRD1 localization at basal conditions (C) and upon 15 minutes (D) of dopamine treatment of SH-SY5Y-DRD1 cells transduced or not by alpha-synuclein WT or A53T. After agonist treatment the cells were fixed and incubated with the different primary antibodies (anti-FLAG for DRD1 and ab133474) and with Alexa647-conjugated secondary antibody (red) or Alexa488-conjugated secondary antibody (green) for DRD1 or LRRK2 respectively. Scale bars = 10μm.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133474).

  • Immunocytochemistry - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
    Immunocytochemistry - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

    Clone MJFF2 (c41-2) (ab172378) has been successfully conjugated by Abcam. This image was generated using Anti-LRRK2 antibody [MJFF2 (c41-2)] (Alexa Fluor® 647). Please refer to ab195023 for protocol details.

    ab195023 staining LRRK2 in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195023 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
    Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

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