Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
![Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)](https://www.abcam.com/ps/products/203/ab203181/Images/ab203181-245525-anti-lrrk2-phospho-s1292-antibody-mjfr-19-7-8-western-blot.jpg "Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8]
See all LRRK2 primary antibodies -
Description
Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose.
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General notes
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJFR-19-7-8 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml
Lane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit (IRDye 800) at 1/10000 dilution
Predicted band size: 286 kDa
Observed band size: 313 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% BSA/TBST.
The image is provided by Dr. Jeremy Nicols, Parkinson’s Institute, Sunnyvale, CA, USA.
G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).
Observed band size: 286 + 27 (GFP) = 313 kDa.
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![Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)](https://www.abcam.com/ps/products/203/ab203181/Images/ab203181-245523-anti-lrrk2-phospho-s1292-antibody-mjfr-19-7-8-western-blot.jpg)
Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml
Lane 1 : LRRK2 mutant G2019S/S1292A transfected HEK293 lysate
Lane 2 : LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysate
Lane 3 : LRRK2 mutant G2019S transfected HEK293 lysate
Lane 4 : LRRK2 mutant S1292A transfected HEK293 lysate
Lane 5 : LRRK2 wt transfected HEK293 inhibitor treated lysate
Lane 6 : LRRK2 wt transfected HEK293 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2500 dilution
Predicted band size: 286 kDa
Observed band size: 286 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
The image is provided by Dr. Paul Davies, University of Dundee, Dundee Scotland, UK.
G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 3).
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![Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)](https://www.abcam.com/ps/products/203/ab203181/Images/ab203181-355302-anti-lrrk2-phospho-s1292-antibody-mjfr-19-7-8-western-blot.JPG)
Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) Kluss et al NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1Characterization of pS1292 LRRK2 antibody
Increasing amounts of HEK-293FT lysates transiently expressing WT LRRK2 (A) or S1292A LRRK2 (B) were analyzed by western blot and probed with the commercially available anti-pS1292 LRRK2 antibody (ab203181). The antibody showed minimal non-specific bands and linear detection in the range tested (b).
From Figure1a of Kluss et al.
Kluss et al NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1
Reproduced under Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/.
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![Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)](https://www.abcam.com/ps/products/203/ab203181/Images/ab203181-5-benefits-of-recombinant-antibodies.png)
Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
