Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292)
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8]
    See all LRRK2 primary antibodies

  • Description

    Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose.

  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    MJFR-19-7-8

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
    Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
    All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml

    Lane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit (IRDye 800) at 1/10000 dilution

    Predicted band size: 286 kDa
    Observed band size: 313 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% BSA/TBST.

    The image is provided by Dr. Jeremy Nicols, Parkinson’s Institute, Sunnyvale, CA, USA.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).

    Observed band size: 286 + 27 (GFP) = 313 kDa.

  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
    Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
    All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml

    Lane 1 : LRRK2 mutant G2019S/S1292A transfected HEK293 lysate
    Lane 2 : LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysate
    Lane 3 : LRRK2 mutant G2019S transfected HEK293 lysate
    Lane 4 : LRRK2 mutant S1292A transfected HEK293 lysate
    Lane 5 : LRRK2 wt transfected HEK293 inhibitor treated lysate
    Lane 6 : LRRK2 wt transfected HEK293 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2500 dilution

    Predicted band size: 286 kDa
    Observed band size: 286 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The image is provided by Dr. Paul Davies, University of Dundee, Dundee Scotland, UK.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 3).

  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
    Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) Kluss et al NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1

    Characterization of pS1292 LRRK2 antibody

    Increasing amounts of HEK-293FT lysates transiently expressing WT LRRK2 (A) or S1292A LRRK2 (B) were analyzed by western blot and probed with the commercially available anti-pS1292 LRRK2 antibody (ab203181). The antibody showed minimal non-specific bands and linear detection in the range tested (b).

    From Figure1a of Kluss et al.

    Kluss et al  NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1

    Reproduced under Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/.

  • Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)
    Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)

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