Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UDD3 30(12)] to LRRK2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free
    See all LRRK2 primary antibodies

  • Description

    Rabbit monoclonal [UDD3 30(12)] to LRRK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab170993 is the carrier-free version of ab133518 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab170993 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
    Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)

    This WB data was generated using the same anti-LRRK2 antibody clone, UDD3 30(12), in a different buffer formulation (cat# ab133518).

    Lane 1: Wild-type A549 cell lysate (20 µg)
    Lane 2: LRRK2 knockout A549 cell lysate (20 µg) 
    Lane 3: Wild-type MEF cell lysate (20 µg)
    Lane 4: LRRK2 knockout MEF cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133518 observed at 238 kDa. Red - loading control, ab130007, observed at 124 kDa.

    ab133518 was shown to specifically react with wild type A549 and MEF cell lines. No band was observed when knock out samples were used. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. Ab133518 and ab130007 (loading control to Vinculin) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    Wild-type and LRRK2 knockout MEF and A549 cells were provide as generous a gift from Professor Dario Alessi, MRC Protein Phosphorylation and Ubiquitination Unit (University of Dundee).

  • Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
    Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)

    Immunofluorescent staining of SH-SY5Y cells fixed and permeablized with 4% PFA and 0.1% Triton X 100 using purified ab133518 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary (ab150077, 1/400) and the sample was stained with DAPI. The negative control is shown in bottom right hand panel - for the negative control, purified ab133518 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133518).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)

    This IHC data was generated using the same anti-LRRK2 antibody clone, UDD3 30(12), in a different buffer formulation (cat# ab133518).

    Immunohistochemical staining of paraffin-embedded human kidney with purified ab133518 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
    Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)

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