HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)
![HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)](https://www.abcam.com/ps/products/195/ab195024/Images/ab195024-311997-anti-lrrk2-antibody-mjff2-c41-2-hrp-western-blot.jpg "HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-LRRK2 antibody [MJFF2 (c41-2)]
See all LRRK2 primary antibodies -
Description
HRP Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293 cells transfected with LRRK2. IHC-P: FFPE human cerebellum (normal) tissue sections.
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General notes
This antibody was developed with support from The Michael J. Fox Foundation.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJFF2 (c41-2) -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)All lanes : HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024) at 1/5000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : LRRK2 knockout A549 whole cell lysate
Lane 3 : Wild-type MEF whole cell lysate
Lane 4 : LRRK2 knockout MEF whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 286 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab195024 was shown to recognize LRRK2 in wild-type cells as signal was lost at the expected MW in LRRK2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. Ab195024 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)](https://www.abcam.com/ps/products/195/ab195024/Images/ab195024-254819-anti-lrrk2-antibody-mjff2-c41-2-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)IHC image of LRRK2 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195024 at 1/500 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)](https://www.abcam.com/ps/products/195/ab195024/Images/ab195024-238365-anti-lrrk2-antibody-mjff2-c41-2-hrp-western-blot.jpg)
Western blot - HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)All lanes : HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 cell lysate transfected with 3*Flag full length wild type LRRK2
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 286 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195024 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)](https://www.abcam.com/ps/products/195/ab195024/Images/ab195024-4-benefits-of-recombinant-antibodies.png)
HRP Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195024)
