Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)
![Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)](https://www.abcam.com/ps/products/193/ab193311/Images/ab193311-243997-anti-gaba-a-receptor-alpha-2-antibody-n399-19-western-blot.jpg "Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)")
Key features and details
- Mouse monoclonal [N399/19] to GABA A Receptor alpha 2/GABRA2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19]
See all GABA A Receptor alpha 2/GABRA2 primary antibodies -
Description
Mouse monoclonal [N399/19] to GABA A Receptor alpha 2/GABRA2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Rat GABA A Receptor alpha 2/GABRA2 aa 350-450.
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Positive control
- ICC/IF: SKNSH cell line.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
This product was previously labelled as GABA A Receptor alpha 2
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N399/19 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)All lanes : Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Pancreas (Rat) Tissue Lysate
Lane 5 : Human pancreas tissue lysate - total protein (ab29816)
Lane 6 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Additional bands at: 104 kDa (possible dimer)
Exposure time: 20 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)](https://www.abcam.com/ps/products/193/ab193311/Images/ab193311-238802-anti-gaba-a-receptor-alpha-2-antibody-n399-19-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311)ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
