Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)
![Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)](https://www.abcam.com/ps/products/109/ab109294/Images/ab109294-122041-anti-ube2n-ubc13-antibody-epr5163-western-blot.jpg "Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5163] to Ube2N / Ubc13
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Ube2N / Ubc13 antibody [EPR5163]
See all Ube2N / Ubc13 primary antibodies -
Description
Rabbit monoclonal [EPR5163] to Ube2N / Ubc13 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HeLa, Raji and 293T cell lysates Flow Cyt: Jurkat cells
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5163 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)All lanes : Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa
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![Flow Cytometry - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)](https://www.abcam.com/ps/products/109/ab109294/Images/ab109294-122040-anti-ube2n-ubc13-antibody-epr5163-flow-cytometry.jpg)
Flow Cytometry - Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)Flow cytometric analysis of Ube2N / Ubc13 in permeabilized Jurkat cells, using ab109294 at a dilution of 1/250 (red) or a rabbit IgG (negative) (green).
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![Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)](https://www.abcam.com/ps/products/109/ab109294/Images/ab109294-3-benefits-of-recombinant-antibodies.png)
Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)
