Anti-Ube2N / Ubc13 antibody (ab228744)
Key features and details
- Rabbit polyclonal to Ube2N / Ubc13
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ube2N / Ubc13 antibody
See all Ube2N / Ubc13 primary antibodies -
Description
Rabbit polyclonal to Ube2N / Ubc13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Cow, Dog, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human Ube2N/ Ubc13 (internal sequence). The exact sequence is proprietary.
Database link: P61088 -
Positive control
- WB: Mouse brain whole cell lysate; A431 and HepG2 whole cell lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab228744)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ube2N / Ubc13 (green) using ab228744 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-Ube2N / Ubc13 antibody (ab228744)Anti-Ube2N / Ubc13 antibody (ab228744) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 17 kDa12% SDS-PAGE
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Western blot - Anti-Ube2N / Ubc13 antibody (ab228744)All lanes : Anti-Ube2N / Ubc13 antibody (ab228744) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDa12% SDS-PAGE
