Anti-Ube2N / Ubc13 antibody (ab25885)
Key features and details
- Rabbit polyclonal to Ube2N / Ubc13
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ube2N / Ubc13 antibody
See all Ube2N / Ubc13 primary antibodies -
Description
Rabbit polyclonal to Ube2N / Ubc13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.
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Positive control
- Human small intestine or HepG2 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purification notes
This antibody was purified by Ion exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ube2N / Ubc13 antibody (ab25885)All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis
in human
small intestine cell lysates 15ug/lane
)
Lane 1 : 0.5 ug/ml of antibody.
Lane 2 : 1 ug/ml of antibody.
Lane 3 : 2 ug/ml of antibody.
Predicted band size: 18 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2N / Ubc13 antibody (ab25885)ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab25885)ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
