Anti-NMDAR2B (phospho Y1252) antibody (ab18532)
Key features and details
- Rabbit polyclonal to NMDAR2B (phospho Y1252)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NMDAR2B (phospho Y1252) antibody
See all NMDAR2B primary antibodies -
Description
Rabbit polyclonal to NMDAR2B (phospho Y1252) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse NMDAR2B aa 1200-1300 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab19636)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)All lanes : ab18532 at 1 mg/ml
Lane 1 : Mouse Brain Tissue Lysate
Lane 2 : Mouse Fore-brain Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Cerebellum Tissue Lysate
Lane 5 : Mouse Hippo-campus Tissue Lysate
Lane 6 : Human Brain Tissue Lysate
Lane 7 : Human Cortex Tissue Lysate
Lane 8 : Human Cerebellum Tissue Lysate
Lane 9 : Human Hippo-campus Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 18 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLanes 1-3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/ml
Lane 4 : NR2B antibody (positive control)
Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/ml
Lane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/ml
Lanes 3-4 : mouse cortical extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References (2)
ab18532 has been referenced in 2 publications.
- Sánchez-Blázquez P et al. Sigma 1 Receptor Antagonists Inhibit Manic-Like Behaviors in Two Congenital Strains of Mice. Int J Neuropsychopharmacol 21:938-948 (2018). PubMed: 29860313
- Delint-Ramirez I et al. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93. J Neurosci 30:8162-70 (2010). PubMed: 20554866
Images
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Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)All lanes : ab18532 at 1 mg/ml
Lane 1 : Mouse Brain Tissue Lysate
Lane 2 : Mouse Fore-brain Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Cerebellum Tissue Lysate
Lane 5 : Mouse Hippo-campus Tissue Lysate
Lane 6 : Human Brain Tissue Lysate
Lane 7 : Human Cortex Tissue Lysate
Lane 8 : Human Cerebellum Tissue Lysate
Lane 9 : Human Hippo-campus Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 18 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532) This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLanes 1-3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/ml
Lane 4 : NR2B antibody (positive control)
Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/ml
Lane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/ml
Lanes 3-4 : mouse cortical extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
