Antibodies, Proteins, Kits and Reagents for Life Science

PE Anti-NMDAR2B antibody [N59/36] (ab274365)

Key features and details

PE Mouse monoclonal [N59/36] to NMDAR2B
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human
Conjugation: PE. Ex: 488nm, Em: 575nm
Isotype: IgG2b

Product name

PE Anti-NMDAR2B antibody [N59/36]
See all NMDAR2B primary antibodies
Description

PE Mouse monoclonal [N59/36] to NMDAR2B
Host species

Mouse
Conjugation

PE. Ex: 488nm, Em: 575nm
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment aa 1-300 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00960

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Positive control
- ICC: HaCat cells. WB: HEK-293T NR2B induced lysate; Rat brain lysate IHC-P: Mouse backskin tissue.
General notes

The clone number has been updated from S59-36 to N59/36, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

N59/36
Isotype

IgG2b
Research areas
- Neuroscience
- Processes

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-NMDAR2B antibody [N59/36] (ab274365)

Immunohistochemistry analysis of Bouin’s Fixative-fixed, paraffin-embedded mouse backskin tissue stained for NMDAR2B (Green) using ab93610 at 1/100 dilution, followed by a FITC-conjugated Goat-Anti-Mouse secondary at 1/50 dilution.

This data was developed using the unconjugated version of this antibody (ab93610).
Western blot - PE Anti-NMDAR2B antibody [N59/36] (ab274365)

All lanes : Anti-NMDAR2B antibody [N59/36] (ab93610) at 1/250 dilution

Lane 1 : NR2B induced HEK-293T lysate
Lane 2 : NR2B non-induced HEK-293T lysate
Lane 3 : Negative control

Predicted band size: 166 kDa

This data was developed using the unconjugated version of this antibody (ab93610).
Immunocytochemistry/ Immunofluorescence - PE Anti-NMDAR2B antibody [N59/36] (ab274365)

Immunocytochemistry/Immunofluorescence analysis of cold 100% methanol-fixed for 10 minutes at -20°C, HaCaT cells stained for NMDAR2B (Green) using ab93610 at 1/100 dilution, followed by a FITC-conjugated Goat Anti-Mouse secondary at 1/50 dilution.

This data was developed using the unconjugated version of this antibody (ab93610).
Western blot - PE Anti-NMDAR2B antibody [N59/36] (ab274365)

Anti-NMDAR2B antibody [N59/36] (ab93610) at 1/1000 dilution + Rat brain membrane tissue lysate at 15 µg

Predicted band size: 166 kDa

This data was developed using the unconjugated version of this antibody (ab93610).

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