Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23460-119] to NMDAR2B - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free
    See all NMDAR2B primary antibodies

  • Description

    Rabbit monoclonal [EPR23460-119] to NMDAR2B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat hippocampus tissue lysate. Rat brain tissue lysate. IHC-P: Human cerebrum tissue. Mouse and rat hippocampus tissue. IP: Mouse and rat brain tissue lysate.

  • General notes

    ab275096 is the carrier-free version of ab254356.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    All lanes : Anti-NMDAR2B antibody [EPR23460-119] (ab254356) at 1/2000 dilution

    Lane 1 : Mouse hippocampus tissue lysate at 10 µg
    Lane 2 : Mouse liver tissue lysate at 20 µg
    Lane 3 : Rat brain tissue lysate at 10 µg
    Lane 4 : Rat hippocampus tissue lysate at 10 µg
    Lane 5 : Rat liver tissue lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 166 kDa



    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    Bands of 180KDa and 115KDa respectively represent the full length and endogenously cleaved form.

    The expression profile observed is consistent with what has been described in the literature (PMID: 22523092)

    Negative control: Liver (PMID: 10049997, 9712644).

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human cerebrum (PMID: 7931566). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    NMDAR2B was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 µg with ab254356 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg.

    Lane 2: ab254356 IP in mouse brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254356 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates. The expression profile observed is consistent with what has been described in the literature (PMID: 22523092).

  • Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Anti-NMDAR2B antibody [EPR23460-119] (ab254356) at 1/1000 dilution + Human hippocampus tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Predicted band size: 166 kDa



    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    Bands of 180KDa, 150KDa and 115KDa respectively represent the full length and endogenously cleaved form.

    The expression profile observed is consistent with what has been described in the literature (PMID: 22523092).

    Exposure time: 48 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse hippocampus (PMID: 7931566). The section was incubated with ab254356 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the rat hippocampus (PMID: 7931566). The section was incubated with ab254356 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

    This data was developed using ab254356, the same antibody clone in a different buffer formulation.

    NMDAR2B was immunoprecipitated from 0.35 mg rat brain tissue lysate 10 µg with ab254356 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 µg.

    Lane 2: ab254356 IP in rat brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254356 in rat brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates. The expression profile observed is consistent with what has been described in the literature (PMID: 22523092).

  • Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)
    Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (ab275096)

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