Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Human Kappa Light Chain antibody [RM126] (ab195576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM126] to Human Kappa Light Chain
  • Suitable for: IHC-P, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-Human Kappa Light Chain antibody [RM126]
    See all Human Kappa Light Chain primary antibodies

  • Description

    Rabbit monoclonal [RM126] to Human Kappa Light Chain

  • Host species

    Rabbit

  • Specificity

    ab195576 reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG.

  • Tested applications

    Suitable for: IHC-P, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Human Kappa Light Chain. Human IgG purified from Human plasma.

  • Positive control

    • Human tonsil tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab195576 was purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM126

  • Isotype

    IgG

  • Research areas

Images

  • ELISA - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
    ELISA - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)

    The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 10 µg/ml and plasma/serum at 1:10 and gradually diluted 1:4, 50µl/well for 2h. Ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)

    Immunohistochemical analysis of Human tonsil tissue labeling Human IgG Kappa Light Chain with ab195576 at 2 µg/ml.

  • Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
    Anti-Human Kappa Light Chain antibody [RM126] (ab195576)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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