Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
![Anti-Human Kappa Light Chain antibody [RM126] (ab195576)](https://www.abcam.com/ps/products/195/ab195576/Images/ab195576-257617-anti-human-kappa-light-chain-antibody-rm126-elisa.jpg "Anti-Human Kappa Light Chain antibody [RM126] (ab195576)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM126] to Human Kappa Light Chain
- Suitable for: IHC-P, ELISA
- Reacts with: Human
Overview
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Product name
Anti-Human Kappa Light Chain antibody [RM126]
See all Human Kappa Light Chain primary antibodies -
Description
Rabbit monoclonal [RM126] to Human Kappa Light Chain -
Host species
Rabbit -
Specificity
ab195576 reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG. -
Tested applications
Suitable for: IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Human Kappa Light Chain. Human IgG purified from Human plasma.
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Positive control
- Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab195576 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM126 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 10 µg/ml and plasma/serum at 1:10 and gradually diluted 1:4, 50µl/well for 2h. Ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)](https://www.abcam.com/ps/products/195/ab195576/Images/ab195576-232882-anti-human-kappa-light-chain-antibody-rm126-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)Immunohistochemical analysis of Human tonsil tissue labeling Human IgG Kappa Light Chain with ab195576 at 2 µg/ml.
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![Anti-Human Kappa Light Chain antibody [RM126] (ab195576)](https://www.abcam.com/ps/products/195/ab195576/Images/ab195576-5-benefits-of-recombinant-antibodies.png)
Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
